Effect of protein depletion on the RNA content and proteosynthesis level in rat liver and brain.

The authors studied the effect of protein depletion on the RNA content of rat liver and brain and on the incorporation of 14C-leucine into the microsomal and soluble liver and brain fractions. They failed to find a direct relationship between the amount of RNA and the rate of proteosynthesis in the organs in question. The changes found in 14-C-leucine incorporation are discussed in relation to previous studies.     

Effect of brain, liver and muscle cytosols on in vitro incorporation of labelled amino acids into the ribosomes of these organs during protein depletion.

The functional state of the rat liver, brain and muscle proteosynthetic apparatus during developing protein depletion was studied, with special reference to the effect of brain cytosols on the synthetic capacity of liver and muscle ribosomes. The results indicate that both stimulant and inhibitory agents (so far unidentified) may be present in brain cytosols during protein depletion.     

Impairment of protein biosynthesis in the spinal cord in ischemia and recirculation

Polysomic profiles and ultrastructure of granulated endoplasmic reticulum of the dog spinal cord as well as incorporation of labelled amino acids to cytosol proteins during ischemia and recirculation are studied. Ischemia does not evoke serious changes of the studied parameters while several minutes after ligature removal there appear pronounced changes in the ribosome distribution confirmed by electron-microscopic examination. Complete restoration of the observed parameters after the induced ischemia requires two days.